Immobilization of DNA for affinity chromatography and drug-binding studies

comparative dna interaction studies of antiviral drug, zidovudine and its complex using different instrumental methods

هدف از این مطالعه بررسی امکان استفاده از داروهای شناخته شده در درمان سایر بیماریها به عنوان داروهای ضد سرطان است. همچنین با استفاده از این داروها در ساختمان کمپلکس فلز می توان شاخص های دارویی بدست آمده را بررسی نمود. داروی ضد ویروس ایدز(hiv)به نام زیدوودین(azt)انتخاب و.کمپلکس.محلول.در.آب[pt(azt)2]cl2سنتزو به روشهای مختلف فیزیکی و شیمیایی شناسایی گردید. بر هم کنش مقایسه ای این دارو و کمپلکس پلا...

Affinity Chromatography in Environmental Analysis and Drug-Protein Interaction Studies

Purification of sequence-specific DNA-binding proteins by affinity chromatography.

The affinity chromatography procedure described in this unit uses DNA containing specific recognition sites for the desired protein that has been covalently linked to a solid support. Preparation of a DNA affinity resin, including cyanogen bromide (CNBr) activation of the agarose support, is described, and an alternate protocol provides a method to couple DNA to commercially available CNBr-acti...

BINDING OF THE ANTITUMOR DRUG ADRIAMYCIN TO DNA-HISTONE COMPLEXES

Isotherms of the binding of the anthracycIine antibiotic, adriamycin (adriblastin), to DNA histone complexes was studied by means of spectroscopic analysis. The results indicated that: (a) binding of adriamycin to histones reduced the interaction of histones with DNA, (b) binding of the drug to DNA did not change the binding affinity of histone to DNA and, (c) in the explored binding range...

Studies on the Tryptophan and Drug-binding Properties of Human Serum Albumin Fragments by Affinity Chromatography and Circular Dichroism Measurements*

After cyanogen bromide digestion of human serum albumin, three fragments, A, B, and C, are obtained. Two different types of a.fEnity columns of Sepharose with L-tryptophan were prepared, one with a free carboxyl group and one with a free amino group. Only Fragment C showed affinity to the Sepharose column with the carboxyl group of tryptophan free, while the column having the amino group free d...